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Journal of Structural Biology
Volume 125, Issue 2-3, April 1999, Pages 133-155
  
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DOI: 10.1006/jsbi.1999.4097
Document Type: Article

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Video on the Internet: An introduction to the digital encoding, compression, and transmission of moving image data

Boudier, T.Show author detailsEmail this author, Shotton, D.M.Show author detailsEmail this author Correspondence address

Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, United Kingdom

Abstract

In this paper, we seek to provide an introduction to the fast-moving field of digital video on the Internet, from the viewpoint of the biological microscopist who might wish to store or access videos, for instance in image databases such as the BioImage Database (http://www.bioimage.org). We describe and evaluate the principal methods used for encoding and compressing moving image data for digital storage and transmission over the Internet, which involve compromises between compression efficiency and retention of image fidelity, and describe the existing alternate software technologies for downloading or streaming compressed digitized videos using a Web browser. We report the results of experiments on video microscopy recordings and three- dimensional confocal animations of biological specimens to evaluate the compression efficiencies of the principal video compression-decompression algorithms (codecs) and to document the artefacts associated with each of them. Because MPEG-1 gives very high compression while yet retaining reasonable image quality, these studies lead us to recommend that video databases should store both a high-resolution original version of each video, ideally either uncompressed or losslessly compressed, and a separate edited and highly compressed MPEG-1 preview version that can be rapidly downloaded for interactive viewing by the database user.

Author Keywords

BioImage; Codec; Compression; Database; Decompression; Digital; Image; Internet; JPEG; Microscopy; Movie; MPEG; Streaming; Video; VIDOS; WWW

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Correspondence address Shotton, D.M.; Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom; email:david.shotton@zoo.ox.ac.uk
© Copyright 2007 Elsevier B.V., All rights reserved.


Journal of Structural Biology
Volume 125, Issue 2-3, April 1999, Pages 133-155
  
 
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